Category: Science News@RCB

Ancestral Variations of the PCDHG Gene Cluster Predispose to Dyslexia in a Multiplex Family

Dyslexia is a heritable neurodevelopmental disorder characterized by difficulties in reading and writing. In the present collaborative study, a strong correlation was observed between variations in the protocadherin gamma gene cluster and dyslexia in the members of one family.The protocadherins are trans synaptic molecules involved in cell-cell recognition in the human brain especially in the […]

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Divided we conquer: The curious case of a bipartite bacterial effector

Plant-pathogen interactions are constant warfare where in most cases the counter-evolution of the latter is more rapid than the invaded plant-kind. This causes most biotechnological approaches including engineered transgenic plants to fail in their defense sustainability. Recent advances in understanding of functions of pathogenic effectors have greatly highlighted this phenomenon. The fundamental role of effectors […]

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Nitrene insertion into an adjacent o-methoxy group followed by nucleophilic addition to the heterocumulene intermediate: Experimental and computational studies

Aryl azides are considered as “green” reagents as most of their reactions involve only a benign loss of nitrogen. On the other hand, in the ‘Click’ reaction all the three nitrogen atoms are retained. Aryl azides are very reactive and many short lived intermediates are generated by both thermal and photochemical decomposition of aryl azides. […]

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New Small Heterobifunctional Cross-linkers for Studying Protein-Protein interactions: Use of ESI-MS and STAVRO X 3.6.0.1 a Bioinformatics Software, Especially Suited for Identifying Intermolecular Crosslinking.

New small N-hydroxysuccinimide (NHS)-aryl azide based heterobifunctional crosslinkers have been used as a ‘proof-of-concept’ to crosslink Lysozyme, in a two-step protocol, involving an initial incubation step followed by photolysis (366 nm, 6W lamp). This was further subjected to SDS-PAGE, excision of the ‘dimeric’ band, trypsin digestion and analyzed by ESI-MS and StavroX 3.6.0.1, a bioinformatics […]

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Parkinson’s disease associated α-synuclein oligomers causes cellular membrane damage and toxicity

α-Synuclein, a major constituent of proteinaceous inclusions named Lewy body, has been shown to be released and taken up by cells, which may facilitate its progressive pathological spreading and neuronal cell death in Parkinson’s disease. However, the pathophysiological effect and signalling cascade initiated by extracellular α-synuclein in cellular milieu are not well understood. Herein we […]

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