Molecular Approaches for Analysis of Drosophila MicroRNAs

MicroRNAs (miRNAs) belong to a class of small non-coding endogenous RNAs that regulate gene expression at the post-transcriptional level. These small RNAs recognize sequences within 3′ untranslated regions of target mRNAs in complexes referred to as a miRNA-induced silencing complex (miRISC). Drosophila melanogaster has served as an indispensable model system for defining the diverse biological roles of miRNAs, their mechanism of action as well as the role of miRNA biogenesis factors. In this chapter, Dr. Geetanjali Chawla’s laboratory describes some of the assays used for molecular analysis of Drosophila miRNAs.

Full article: https://link.springer.com/protocol/10.1007%2F978-1-4939-9756-5_14