Obligate biotrophic fungal pathogens establish long-term feeding relationships with their plant host, during which they siphon sugars from host cells through haustoria. The Arabidopsis hexose transporter, STP13 (sugar transport protein 13), was previously shown to confer resistance against hemi-biotrophic and necrotrophic pathogens by limiting sugar flux towards these pathogens. By contrast, expression of Lr67res, a […]

The X family is one of the eight families of DNA polymerases (dPols) and members of this family are known to participate in the later stages of Base Excision Repair. Many prokaryotic members of this family possess a Polymerase and Histidinol Phosphatase (PHP) domain at their C-termini. The PHP domain has been shown to possess […]

 Microtubule-associated protein 1 light chain 3 (MAP1LC3) is a protein with a well-defined function in autophagy, but still incompletely understood roles in several other autophagy-independent processess. Studies have shown MAP1LC3 is a host dependency factor for the replication of several viruses. Japanese encephalitis virus (JEV), a neurotropic flavivirus, replicates on ER derived membranes that are […]

Multiple membrane trafficking networks operate in the eukaryotic cell and are hijacked by viruses to establish infection. Recent studied have highlighted that viruses can exploit distinct pathways depending on the cell type. Japanese encephalitis virus (JEV), a neurotropic flavivirus, can infect neuronal cells through a clathrin-independent endocytic mechanism. To further characterize the membrane trafficking requirements […]

Transient gene expression is an important tool for deciphering molecular functions of a gene in a very short period. It is useful for studying the intracellular localization of proteins, to uncover in-vivo protein-protein, protein-DNA and protein-RNA interactions, and to perform Co-IP, ChIP and protein activity experiments. Although this method is extensively employed for plant species […]