Comparative analysis of miRNAs landscape of exosomes from different sources of mesenchymal stem cells: Implication in immune cells function and on hepatoprotective capacity.

Adult Mesenchymal stem cells(MSCs) -derived exosomes carry several biologically active molecules. Depending on their origin, the content of their cargo and their functions may vary. This study aimed to get more in-depth insights into the differences and similarities between exosomes isolated from different origins i.e., bone marrow (BM), adipose tissue (AD), and Wharton Jelly (WJ), in the context of their miRNA landscape. It also studied the differences in immunomodulatory roles of these exosomes from other sources. Our study showed that depending on the source of exosomes, these exosomes demonstrated their varying effect on PBMC proliferation, neutrophil survival, and NET formation, highlighting their versatility and broad interaction with immune cells.
We also documented the differential regenerative efficacy of extracellular vesicles (EVs) derived from two different human tissue-specific MSCs (Adipose tissue; AD-MSC and Wharton’s Jelly; WJ-MSC) in a murine model of chronic liver fibrosis. AD-MSC EVs exhibited superior anti-fibrotic activity over WJ-MSC extracellular vesicles. A panel of liver regenerative miRNA and proteins was present in EVs that were upregulated in AD-MSC EVs.

MicroRNA-Enriched Exosomes from Different Sources of Mesenchymal Stem Cells Can Differentially Modulate Functions of Immune Cells and Neurogenesis. Biomedicines 2022, 10(1), 69; https://doi.org/10.3390/biomedicines10010069

Comparative Evaluation of Anti-Fibrotic Effect of Tissue-Specific Mesenchymal Stem Cells Derived Extracellular Vesicles for the Amelioration of CCl4 Induced Chronic Liver Injury. Stem Cell Rev Rep. 2021 Dec 2. doi: 10.1007/s12015-021-10313-9