Purification of exosome-enriched proteins produced in a Drosophila cell line by size exclusion chromatography

Exosomes are a class of extracellular vesicles that play a role in intercellular signaling under diverse contexts. Here we describe a protocol that has been optimized for the isolation and characterization of exosomes from a Drosophila melanogaster cell line using size exclusion chromatography (SEC). The specific focus of this protocol was to examine the starvation-induced exosome-loading of a protein.

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