Purification of exosome-enriched proteins produced in a Drosophila cell line by size exclusion chromatography

Exosomes are a class of extracellular vesicles that play a role in intercellular signaling under diverse contexts. Here we describe a protocol that has been optimized for the isolation and characterization of exosomes from a Drosophila melanogaster cell line using size exclusion chromatography (SEC). The specific focus of this protocol was to examine the starvation-induced exosome-loading of a protein.

 

 

 

https://star-protocols.cell.com/

Share

Leave a Reply

Your email address will not be published. Required fields are marked *

Time limit is exhausted. Please reload the CAPTCHA.